Selection of bacteria suitable for introduction into the rhizosphere of grass
Nijhuis, E.H. | Maat, M.J. | Zeegers, I.W.E. | Waalwijk, C. | Veen, J.A. van.
A procedure was developed to select bacteria suitable for introduction into the rhizosphere of grass. The aim was to find a natural inhabitant of the grass rhizosphere, with a good root colonization capacity and survival upon introduction, without a specific preference for the relatively young or old part of the root system. The composition of the natural bacterial population of a grass rhizosphere was determined in order to find the main taxonomical groups. Isolates were tested on root colonization capacity, using grass seedlings on agar plates. Based on the results, 16 strains, belonging to the main taxonomical groups isolated from the grass rhizosphere, were selected to study root colonization in sterile soil. Possible preference of the strains for either the root tip or base was also determined. Then, 7 strains were selected to study root colonization, preference and survival in natural soil. Finally, survival and colonization capacity of a selected Pseudomonas cepacia strain P2 was studied after introduction in a field experiment, in order to verify previous results and selection criteria. The population size of the introduced strain P2 remained stable for 10 wk in the rhizosphere of grass. These results suggested that the present procedure was useful in the selection of suitable bacteria for introduction in the rhizosphere.Show more [+] Less [-]