Method to minimize ozone effect on Cy5 fluorescent intensity in DNA microarrays
2017
Kim, Youngjun | Seo, Hyunhee | Jeong, Miseon | Lee, Kiheon | Lee, Inho | So, Kyeonga | Kim, Mikyung | Lee, Yookyung | Kim, Seonah | Kim, Taejin
Cyanine 5 (Cy5) is an established fluorescent dye in microarray analysis. It is degraded rapidly when exposed to atmospheric ozone during post-hybridization washes, which leads to loss of fluorescent intensity. To minimize this undesirable effect, we coated microarray slides with sodium dodecyl sulfate (SDS) solution at post-hybridization washes. The fluorescent intensities on coated slides were more stable than those on uncoated slide. We also performed the microarrays with SDS solution for a year to check the solution's effectiveness along with seasonal changes of atmospheric ozone level. Consistent results in microarray analysis were obtained using Cy5 dye under atmospheric ozone.
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