14-Deoxyecdysteroids in an Insect (Gryllus bimaculatus)
2014
Hoffmann, Klaus H. | Thiry, Edeltraud | Lafont, Rene
Following injection or ingestion of [³H]ecdysone and [³H]₂O-hydroxyecdysone, respectively, considerable quantities of 14-deoxyecdysteroids could be extracted from gut and faeces of female adult crickets. The compounds were identified as 14-deoxyecdysone and 14-deoxy-20-hydroxyecdysone on the basis of comigration with reference substances in two HPLC systems and by its mass spectrum (only 14-deoxy-20-hydroxyecdysone). The 14-dehydroxylation is most probably caused by bacteria from the gut lumen. Feeding of [³H]ecdysone resulted in the appearance of labelled 14-deoxyecdysone also in ovaries, fat body, muscles, and rest of the body.
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