Proteome analysis and thermal-tolerant protein marker screening in the skin mucus of large yellow croaker Larimichthys crocea
2021
Zhang, Jing | Cai, Xiaofang | Zhang, Xiaoying | Lin, Longshan | Zhao, Hongbo | Liu, Xiande
Fish skin mucus serves as the first line of defense against pathogens and external stressors. To screen thermal-tolerant protein marker in the skin mucus of large yellow croaker Larimichthys crocea, this study conducted proteome analysis on skin mucus between thermal-tolerant and thermal-sensitive groups of large yellow croaker. A total of 396 differentially expressed proteins (DEPs) were identified, among which 215 proteins were upregulated and 181 proteins were downregulated. Ten selected random DEPs were validated using a parallel reaction monitoring (PRM) assay, and the results showed that the proteome analysis results were reliable (r = 0.805, P < 0.01). The bioinformatics analysis indicated that the proteins with significant changes were involved in immune defense, energy metabolism and heat shock, in which lily-type lectin, plasma protease C1 inhibitor, thrombospondin-1, calreticulin, pyruvate kinase and heat shock protein 70 played important roles in the course of adaption to high temperature and can be used as candidate thermal-tolerant protein markers in large yellow croaker. The results of this study provide a reference for constructing a noninvasive detection technology for assisting in the breeding of thermal-tolerant strains of large yellow croaker in the future.
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