Molecular cloning and expression analysis of interferon-inducible transmembrane protein 1 in large yellow croaker Pseudosciaena crocea
2008
Wan, Xiang | Chen, Xinhua
In human cells, interferon-inducible transmembrane protein 1 (IFITM1) is a component of protein complexes involved in homotypic adhesion and the transduction of antiproliferative signals. Here, we reported the cloning of an IFITM1 homologue from the spleen of large yellow croaker Pseudosciaena crocea (LycIFITM1). The complete cDNA of LycIFITM1 is 734 nucleotides (nt) encoding a protein of 124 amino acids (aa), with a putative molecular weight of 13.6kDa. The deduced LycIFITM1 protein is significantly homologous to interferon-inducible transmembrane proteins (IFITMs) in mammals and fish, and has the typical structural features of IFITMs, including two transmembrane domains (residues 43-63 and 90-112, respectively) and one intracellular domain between them (residues 64-89), as well as one conserved protein kinase C (PKC) phosphorylation site (residues 65-67, SIK). Phylogenetic analysis showed that LycIFITM1 formed a cluster with fish IFITM, reflecting a relative distant evolutionary relationship from mammals. LycIFITM1 gene was constitutively expressed in various tissues examined, such as gills, intestine, liver, kidney, heart, spleen, muscle and blood. Upon induction with poly(I:C), LycIFITM1 gene expression was obviously up-regulated in gills, kidney, heart and spleen at 24h after stimulation, suggesting that LycIFITM1 may be involved in the immune response induced by poly(I:C). Time course analysis using real-time PCR showed that the mRNA levels of LycIFITM1 in spleen and kidney were quickly up-regulated by poly(I:C) and reached the peak at 24h post-induction (48.7- and 280.4-fold mRNA increases in spleen and kidney, respectively). The results suggest that the IFITM1 homologue from large yellow croaker may represent a novel member of IFITMs family in fish.
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