Quality Evaluation of Angelica sinensis by Simultaneous Determination of Ten Compounds Using LC-PDA
2009
Lü, Jie-Li | Zhao, Jing | Duan, Jin'ao | Yan, Hui | Tang, Yu-Ping | Zhang, Lai-Bin
A simple, rapid and valid analysis method for simultaneously determination of ten characteristic components, including six phthalides [(Z)-ligustilide, (Z)-butylidene phthalide, riligustilide, levistolide A, (3Z)-(3aR,6S,3'R,8'S)-3a.8',6.3'-diligustilide and 3,9-dihydroxyl-ligustilide], three aromatic acids (o-phthalic acid, vanillic acid and ferulic acid) and one macrolide (brefeldin A), in the radix of Angelica sinensis, a valued traditional Chinese medicinal herb was developed by liquid chromatography coupled with photodiode array detection. Riligustilide, levistolide A, (3Z)-(3aR,6S,3'R,8'S)-3a.8',6.3'-diligustilide, 3,9-dihydroxyl-ligustilide and brefeldin A were first quantitatively determined in Angelica sinensis. Separation was performed on an Alltima C-18 analytical column by gradient elution with (A) 0.2% aqueous formic acid and (B) methanol as mobile phase. The column temperature was set at 25 °C. The method was validated for system suitability, precision accuracy and linearity. The average recovery of the method was 93.8-102.2% and linearity was (R ² > 0.998). Furthermore, the method was successfully applied to the simultaneous determination of ten analytes in 46 samples of A. sinensis from various sources including different collecting locations, harvesting time, storage time, drying methods, and medicinal portions of the herb. The results obtained suggest that the method can efficiently evaluate the quality of A. sinensis from different sources, which benefit its quality control.
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