RNA binding specificity of a Drosophila snRNP protein that shares sequence homology with mammalian U1-A and U2-B" proteins

Harper, D.S.; Fresco, L.D.; Keene, J.D.

RNA binding specificity of a Drosophila snRNP protein that shares sequence homology with mammalian U1-A and U2-B" proteins

1992

Harper, D.S. | Fresco, L.D. | Keene, J.D.

We have characterized a recombinant Drosophila melanogaster RNA binding protein, D25, by virtue of its antigenic relationship to mammalian U1 and U2 small nuclear ribonucleoprotein (U snRNP) proteins. Sequence analysis revealed that D25 bears strong similarity to both the human U1 snRNP-A (U1-A) and U2 snRNP-B" (U2-B") proteins. However, at residues known to be critical for the RNA binding specificities of U1-A and U2-B" D25 sequence is more similar to U2-B". Using direct RNA binding assays D25 selected U1 RNA from either HeLa or Drosophila Kc cell total RNA. Furthermore, D25 bound U1 RNA when transfected into mammalian cells. Thus, D25 appears to be a Drosophila homolog of the mammalian U1-A protein, despite its sequence similarity to U2-B".

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AGROVOC Keywords

amino acid sequences animals binding proteins chemistry cloning complementary dna drosophila melanogaster escherichia coli genetics humans metabolism nucleoproteins rna rna

Bibliographic information

Published in

Nucleic acids research

Volume 20 Issue 14 Pagination 3645 - 3650 ISSN 0305-1048

Other Subjects

Molecular sequence data; Nucleotide sequences; Rna-binding proteins; Molecular; Amino acid sequence; Small nuclear rna; Hela cells; Western; Blotting; Ribonucleoprotein; U1 small nuclear; Small nuclear; Molecular sequence data; Ribonucleoproteins; Genbank/m89775; Small nuclear; Ribonucleoproteins

Language

English

Note

2019-12-05

Type

Journal Article; Text

In AGRIS since: 2024-02-27

Format: MODS

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