T-bet-dependent regulation of CD8⁺ T-cell expansion during experimental Trypanosoma cruzi infection
2009
Cobb, Dustin | Guo, Siqi | Lara, Ana M. | Manque, Patricio | Buck, Gregory | Smeltz, Ronald B.
The transcription factor T-bet (T-box, expressed in T cells), promotes type I immunity to pathogens through effects involving T cells and dendritic cells. In CD8⁺ T cells, many of the functions of T-bet are redundant with those of eomesodermin (Eomes), a paralogue of T-bet. We therefore investigated the role of T-bet in immunity to Trypanosoma cruzi, an intracellular pathogen that causes Chagas disease, and which requires CD8⁺ T cells for resistance. T-bet-deficient mice (tbx21⁻/⁻) were highly susceptible to T. cruzi infection, marked by severe liver pathology. CD8⁺ T cells from infected tbx21⁻/⁻ mice expressed typical markers of activation, including CD44 and CD25. In striking contrast, there was a 10-fold reduction in the number of antigen-specific CD8⁺ T cells in tbx21⁻/⁻ mice. This reduction was not a consequence of increased apoptosis or altered tissue-specific migration. Further, antigen-presenting cell (APC) functions in tbx21⁻/⁻ mice were normal as we observed comparable levels of B7-1, B7-2 and CD40 expression as well as normal antigen-driven proliferation of wild-type CD8⁺ T cells in infected tbx21⁻/⁻ mice. However, adoptive transfer of naïve T cells from tbx21⁻/⁻ donors into infected Rag-2-deficient mice (tbx21⁺/⁺) demonstrated a similar quantitative defect in CD8⁺ T-cell expansion. These data demonstrate that T-bet facilitates immunity to T. cruzi by promoting the expansion of T. cruzi-specific CD8⁺ T cells in a T cell-intrinsic manner. They also serve to further illustrate the multifaceted functions of T-box proteins in regulating quantitative aspects of T-cell immunity, in addition to qualitative components such as cytokine production.
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