Sequence organization of the Balbiani ring 2.1 gene in Chironomus tentans

Wieslander, L.; Paulsson, G.

Sequence organization of the Balbiani ring 2.1 gene in Chironomus tentans

1992

Wieslander, L. | Paulsson, G.

Balbiani rings are giant chromosomal puffs, containing related genes that provide unique possibilities for in vivo analysis of gene expression at the chromatin and ribonucleoprotein levels. Here, the 5' end of the Balbiani ring 2.1 gene in the dipteran Chironomus tentans has been isolated and the sequence organization of the entire Balbiani ring gene is presented. The gene contains five exons, one being extremely small, only 6 base pairs, and one being extremely large, approximately 30 kilobase pairs. Three introns are located at the 5' end and a fourth one is located at the 3' end. The central 30-kilobase-pair exon is entirely built from tandemly organized repeats. All repeats are virtually identical except for a few variant repeats at both ends of the repeat array. The number of repeats may vary between alleles and the length of the gene therefore changes between 30 and 35 kilobase pairs.

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AGROVOC Keywords

amino acid sequences animals chironomidae chironomus tentans chromosome mapping cloning dna exons exons gene expression genetics introns introns rna

Bibliographic information

Published in

Proceedings of the National Academy of Sciences of the United States of America

Volume 89 Issue 10 Pagination 4578 - 4582 ISSN 0027-8424

Publisher

Taylor & Francis

Other Subjects

Molecular sequence data; Base sequence; Messenger; Chromosome deletion; Nucleotide sequences; Molecular; Isolation & purification; Amino acid sequence; Molecular sequence data; Restriction mapping; Genbank/m89909

Language

English

Note

2019-12-05

Type

Journal Article; Text

In AGRIS since: 2024-02-27

Format: MODS

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