Effects of 20-hydroxyecdysone on protein synthesis in the epithelial cell line from Chironomus tentans
1993
Fretz, A. | Lubbert, A. | Markmann-Mulisch, U. | Spindler-Barth, M. | Spindler, K.D.
In the epithelial cell line of Chironomus tentans growth ceases after addition of 20-hydroxyecdysone. Under the influence of moulting hormone the protein pattern changes as shown by 2-dimensional electrophoresis and silver staining. Incorporation of 35S-labelled methionine reveals qualitative and quantitative changes in the homogenate from the cells and in the proteins secreted into the medium. RNA from cells treated with 20-OH-ecdysone for various times either permanently or with pulses of moulting hormones was expressed in a reticulocyte in vitro translation system. Qualitative changes in the in vitro translated products were first observed after 75 min and continue until day 4 after hormone treatment. A complex pattern of permanent and transient induction and repression of different transcripts is obtained.
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