Computer modeling of two inorganic pyrophosphatases

Vihinen, M.; Lundin, M.; Baltscheffsky, H.

Computer modeling of two inorganic pyrophosphatases

1992

Vihinen, M. | Lundin, M. | Baltscheffsky, H.

The yeast Saccharomyces cerevisiae has two inorganic pyrophosphatases that are structurally related. One, PPA1, is a Cytoplasmic enzyme. The other, PPA2, is located in the mitochondria and appears to be energy-linked. The sequence similarity of PPA1 and PPA2 is about 66% and the identity is about 50%. All amino acids known to be important for catalysis are conserved, except one glutamate which is substituted by an aspartate in PPA2. The structures of PPA2 and the cytoplasmic PPase from Schizosaccharomyces pombe were modeled based on the three dimensional structure of PPA1. Two cysteines in PPA2 and one in the S. pombe enzyme are located at the catalytic cleft. Four residues form an unique insertion near the entrance of the catalytic cleft in the mitochondrial enzyme.

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AGROVOC Keywords

amino acid sequences chemistry cytoplasm databases enzyme activity enzymology genetics isoenzymes mitochondria models models saccharomyces cerevisiae schizosaccharomyces schizosaccharomyces pombe sequence alignment yeasts

Bibliographic information

Published in

Biochemical and biophysical research communications

Volume 186 Issue 1 Pagination 122 - 128 ISSN 0006-291X

Publisher

Elsevier B.V.

Other Subjects

Sequence homology; Nucleic acid; Molecular; Protein conformation; Computer analysis; Factual; Cytosol; Pyrophosphatases; Molecular sequence data; Protein structure; Amino acid sequence; Molecular sequence data; Computer simulation; Inorganic pyrophosphatase; Inorganic pyrophosphatase

Language

English

Note

2019-12-04

Type

Text; Journal Article

In AGRIS since: 2024-02-27

Format: MODS

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