Effect of structural modulation of polyphenolic compounds on the inhibition of Escherichia coli ATP synthase
2012
Ahmad, Zulfiqar | Ahmad, Mubeen | Okafor, Florence | Jones, Jeanette | Abunameh, Abdelmajeed | Cheniya, Rakesh P. | Kady, Ismail O.
In this paper we present the inhibitory effect of a variety of structurally modulated/modified polyphenolic compounds on purified F₁ or membrane bound F₁FₒEscherichia coli ATP synthase. Structural modulation of polyphenols with two phenolic rings inhibited ATP synthase essentially completely; one or three ringed polyphenols individually or fused together inhibited partially. We found that the position of hydroxyl and nitro groups plays critical role in the degree of binding and inhibition of ATPase activity. The extended positioning of hydroxyl groups on imino diphenolic compounds diminished the inhibition and abridged position enhanced the inhibition potency. This was contrary to the effect by simple single ringed phenolic compounds where extended positioning of hydroxyl group was found to be effective for inhibition. Also, introduction of nitro group augmented the inhibition on molar scale in comparison to the inhibition by resveratrol but addition of phosphate group did not. Similarly, aromatic diol or triol with rigid or planar ring structure and no free rotation poorly inhibited the ATPase activity. The inhibition was identical in both F₁Fₒ membrane preparations as well as in isolated purified F₁ and was reversible in all cases. Growth assays suggested that modulated compounds used in this study inhibited F₁-ATPase as well as ATP synthesis nearly equally.
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