Metabolic fate and organ distribution of 13C-3′-sialyllactose and 13C-N-acetylneuraminic acid in wild-type mice – No evidence for direct incorporation into the brain
2020
Galuska, Christina E. | Rudloff, Silvia | Kuntz, Sabine | Borsch, Christian | Reutzel, Martina | Eckert, Günter | Galuska, Sebastian P. | Kunz, Clemens
Milk sialyllactose (SL) and sialic acids (SA) are considered to be crucial for brain composition and development. To investigate their metabolic fate, we administered ¹³C-labelled 3′SL (¹³C-3′SL) and ¹³C-N-acetylneuraminic acid (¹³C-Neu5Ac) to NMRI mice. From per oral and intravenous (i.v.) applications, an organ specific ¹³C-enrichment can be excluded. The ¹³C-enrichment after oral application (o.a.) was lowest in brain tissue and not detectable after i.v. in any organ. The presence of ¹³C-Neu5Ac in urine after the o.a. of both labelled components demonstrated that ¹³C-Neu5Ac was taken up by gut epithelial cells. Because plasma ¹³C-enrichment increased over time, when the oral ¹³C-bolus had reached the lower gastrointestinal tract, an involvement of intestinal epithelial cells and/or gut microbiota in the metabolism of ¹³C-3′SL and/or ¹³C-Neu5Ac could be assumed. Hence, SL or Neu5Ac might influence the gut brain axis by effects within the gastrointestinal tract rather than being directly incorporated into the brain.
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