Studies on the extraction and purification of phytic acid from rice bran
2011
Canan, Cristiane | Cruz, Felipe Tsuruta Lisboa | Delaroza, Fernanda | Casagrande, Rubia | Sarmento, Cleonice Pereira Mendes | Shimokomaki, Massami | Ida, Elza Iouko
The objective of this work was to develop rice bran IRGA 417 phytic acid (PA) extraction and purification techniques. For the extraction of PA, a complete 2⁴ factorial design with triplicates at the central point was used, and the effects of concentration of rice bran and HCl, time and temperature were investigated. During purification, different pH values were tested with addition of 1.5M Na₂CO₃ or 4.0M NaOH. The results obtained by the statistical analysis of the factorial design showed that temperature, time and HCl concentration influenced the PA extraction technique significantly (p≤0.05), whereas the concentration of rice bran had no influence. The content of PA was evaluated in all the stages of purification and it was possible to establish an improved methodology of extraction and purification with high purity and yields.
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