Integrated transcriptome and metabolome analysis reveals the physiological and molecular responses of allotetraploid rapeseed to ammonium toxicity
2021
Li, Shuang | Yan, Lei | Riaz, Muhammad | White, Philip J. | Yi, Ceng | Wang, Sheliang | Shi, Lei | Xu, Fangsen | Wang, Chuang | Cai, Hongmei | Ye, Xiangsheng | Ding, Guangda
Ammonium (NH₄⁺) and nitrate (NO₃⁻) are the main nitrogen (N) sources accessible to plants. However, exclusive NH₄⁺ may causes toxicity to crops. Transcriptomics in conjunction with metabolomics analysis in allotetraploid rapeseed under NH₄⁺ stress showed that NH₄⁺ taken up by roots was mainly transferred to leaves for assimilation. The concentrations of most amino acids, glucose, and glucose-6-phosphate increased whilst malate, succinate, and α-ketoglutarate concentrations decreased in NH₄⁺-treated leaves. Moreover, NH₄⁺ toxicity down-regulated genes related to photosynthesis and impaired the electron transport chain in chloroplasts. The expression of genes related to H₂O₂ decomposition, as well as the concentrations of monodehydro-ascorbic acid and ascorbic acid, reduced after exposure to NH₄⁺-N, accompanied by higher O₂- and H₂O₂ accumulation. Taken together, our results indicated that the excessive accumulation of NH₄⁺ in plants caused an imbalance of carbon and N metabolism, impaired photosystems and generated reactive oxygen species, which resulted in toxicity to rapeseed.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library