Inhibition of DNA oxidative damage and antimutagenic activity by dichloromethane extract of Brassica rapa var. rapa L. seeds
2015
Robin, | Arora, Saroj | Vig, Adarsh Pal
The current study was designed to evaluate the inhibition of DNA oxidative damage and antimutagenic activity by dichloromethane extract of Brassica rapa var. rapa L. (Turnip) seeds belonging to family Brassicaceae, via DNA oxidative damage assay and Ames assay. The hydroxyl radical mediated oxidative damage of DNA was effectively inhibited by the seed oil extract at 1000μg/ml concentration. The extract was assessed for its antimutagenic activity against direct acting mutagens (sodium azide and 4 nitro-o-phenylenediamine) and indirect acting mutagen (2-aminofluorene), with TA100 and TA98 strain of Salmonella typhimurium. It was found that there was more significant antimutagenic effect against S9 dependent indirect acting mutagen than direct acting mutagens. The results showed better desmutagenic effect with pre-incubation mode of experimentation, as compared to the co-incubation mode, with lowest value of IC50 0.0008μg/0.1ml/plate with TA98 strain against S9 dependent mutagen. The GC–MS analysis revealed the presence of two major compounds, namely phenylethylbrassinin and 3-phenylpropionitrile, in the seed oil extract. These compounds might be the main contributors for inhibition of hydroxyl radical mediated DNA strand breaking and antimutagenic effect by extract. The study indicates the presence of strong antimutagenic factors and hydroxyl radical scavengers in Turnip seeds enabling its possible therapeutic effect. Also, to the extent of our knowledge this is the first study that enlightens the antimutagenic effects of extracted seed oil from B. rapa var. rapa L.
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