Ultrasonic-assisted liquid–liquid extraction and HILIC–ELSD analysis of ginsenoside Rb₁, astragaloside IV and dulcitol in sugar-free “Fufangfufangteng Heji”
2011
Qin, Jieping | Feng, Jun | Li, Yaohua | Mo, Kefeng | Lu, Shiying
A simple method for the extraction and determination of ginsenoside Rb₁, astragaloside IV and dulcitol in sugar-free “Fufangfufangteng Heji” was developed using an ultrasonic-assisted liquid–liquid extraction (UALLE) coupled with Hydrophilic Interaction Liquid Interface Chromatography and Evaporative Light Scattering Detector (HILIC–ELSD) analysis. Good chromatographic separation was achieved using a Phenomenex Luna HILIC column (250mm×4.6mm i.d., 5μm), and a mobile phase consisting of acetonitrile–water at a flow rate of 1.0ml/min with a gradient elution within 25min was also used. Compared to the conventional analysis method, the proposed method had the advantages of a longer column life, shorter analysis time, lower baseline noise, short sample pretreatment time and low consumption of organic solvent. The linear ranges for ginsenoside Rb₁, astragaloside IV and dulcitol were 0.0256–0.179, 0.110–0.770, 0.105–0.630mg/ml, respectively. The recoveries of ginsenoside Rb₁, astragaloside IV and dulcitol during the pharmaceutical preparation were within the range of 97.2–100.3%, and their relative standard deviations were 1.2–3.1%.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library