Cytochrome P450 genes expressed in the deltamethrin-susceptible and -resistant strains of Culex pipiens pallens
2003
Shen, Bo | Dong, Hui-Qin | Tian, Hai-Sheng | Ma, Lei | Li, Xiu-Lan | Wu, Guan-Liang | Zhu, Chang-Liang
cDNA sequences encoding cytochrome P450 were amplified, respectively, from deltamethrin-susceptible and -resistant strains of the mosquito, Culex pipiens pallens, with a pair of degenerate primers by RT-PCR and the Direct Cloning Method, and then identified by cDNA chip and Northern blot. Twenty-four positive clones obtained were shown to be new sequences. They had been placed in GenBank and were approved by the Cytochrome P450 Nomenclature Committee, classified to the subfamilies CYP4C, CYP4D, CYP4H, and CYP4J. The microarray signal values of five P450 sequences (CYP4H21, 4H22v1, 4J4v2, 4J6v1, and 4J6v2) were 3.1–7.7 times higher in resistant probe than in susceptible probe and the sequence 4H23v2 reacts only with resistant probe. The results of Northern blot of the above six P450 sequences were similar to that of microarray analyses. These results indicate that CYP4 may be related to deltamethrin resistance in Cx. pipiens pallens.
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