Biosynthesis and industrial applications of α-amylase: a review
2021
Farooq, Muhammad Adeel | Tareen, Shaukat | Hassan, Ali | T̤āhir, Ḥāfiz̤ Muḥammad | Mumtaz, Samaira | Mumtaz, Shumaila
Amylase is amongst the most indispensable enzymes that have a large number of applications in laboratories and industries. Mostly, α-amylase is synthesized from microbes such as bacteria, fungi and yeast. Due to the high demand for α-amylase, its synthesis can be enhanced using recombinant DNA technology, different fermentation methods, less expensive and good carbon and nitrogen sources, and optimizing the various parameters during fermentation, e.g., temperature, pH and fermentation duration. Various methods are used to measure the production and activity of synthesized α-amylase like iodine, DNS, NS and dextrinizing methods. The activity of crude α-amylase can be elevated to the maximum level by optimizing the temperature and pH. Some metals also interact with α-amylase and increase its activity like K⁺, Na⁺, Mg²⁺ and Ca²⁺. Some industries such as starch conversion, food, detergent, paper, textile industries and fuel alcohol production extensively utilize α-amylase for their various purposes.
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