A doubly responsive probe for the detection of Cys4-tagged proteins
2015
Kotera, N. | Dubost, E. | Milanole, G. | Doris, E. | Gravel, E. | Arhel, N. | Brotin, T. | Dutasta, J.-P. | Cochrane, J. | Mari, E. | Boutin, C. | Léonce, E. | Berthault, P. | Rousseau, B.
Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized ¹²⁹Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the ¹²⁹Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.
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