Isolation and characterization of a novel biosurfactant produced by hydrocarbon-degrading bacterium Alcanivorax dieselolei B-5
2010
Qiao, N. | Shao, Z.
Our goal was to identify a novel biosurfactant produced by a marine oil-degrading bacterium. Biosurfactants were produced by Alcanivorax dieselolei strain B-5T growing with diesel oil as the sole carbon and energy source. Culture supernatant was first extracted with chloroform/methanol (1 : 1, v/v), then further purified step by step with a normal phase silica gel column, a Sephadex LH20 gel column and a preparative thin layer plate. The main component was determined to be a lipopeptide; it was chemically characterized with nuclear magnetic resonance, liquid chromatography-quadrupole ion-trap mass spectrometry, amino acid analysis and GC-MS and was found to be a mixture of proline lipids. The monomers of the proline lipids were composed of a proline residue and a fatty acid (C₁₄:₀, C₁₆:₀ or C₁₈:₀). The critical micelle concentration of the mixed proline lipids was determined to be 40 mg l⁻¹. Moreover, activity variations in ranges of pH, temperature and salinity were also detected and showed reasonable stability. Alcanivorax dieselolei B-5 produced a novel linear lipoamino biosurfactant, characterized as a proline lipid. A proline lipid was characterized for the first time as a bacterial biosurfactant. This product has potential in both environmental and industrial applications.
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