Antioxidant activity of sage (Salvia officinalis and S fruticosa) and oregano (Origanum onites and O indercedens) extracts related to their phenolic compound content
2002
Pizzale, Lorena | Bortolomeazzi, Renzo | Vichi, Stefania | Uberegger, Eva | Conte, Lanfranco S.
In this study the antioxidant activity of methanolic extracts of oregano and sage samples was tested. Samples of oregano belonged to Origanum onites and O indercedens species, whilst samples of sage belonged to Salvia officinalis and S fruticosa species. Two methods were used to evaluate the antioxidant activity of sage and oregano extracts: the crocin test and the Rancimat test. The methanolic extracts were also analysed by HPLC for the qualitative/quantitative determination of phenolic compounds. The total phenolic compound content of oregano samples showed no significant differences between the two species, but rosmarinic acid was present in higher amount in O indercedens. Carvacrol content sharply differentiated flowers from leaves. Samples of O indercedens had a higher antioxidant activity evaluated by the crocin test, whereas no differences were evidenced by the Rancimat test. For sage samples, carnosic acid and methyl carnosate showed a significant difference between the two species, with S fruticosa samples having a higher content than S officinalis samples. Samples of S fruticosa also had a higher antioxidant activity evaluated by the crocin test. The antioxidant activities of sage samples were, on average, higher than those of oregano samples. Some samples of sage had a very high antioxidant activity, with induction times more than 10-fold higher than that of lard used as the reference sample.
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