Isolation and determination of the activity of IgA1 protease from Neisseria meningitidis
2010
Yagudaeva, E. Yu | Zhigis, L. S | Razgulyaeva, O. A | Zueva, V. S | Melnikov, E. E | Zubov, V. P | Kozlov, L. V | Bichucher, A. M | Kotel'nikova, O. V | Alliluev, A. P | Avakov, A. E | Rumsh, L. D
A method of the isolation and purification of IgA1 protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and precipitate obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been developed. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 µg. It was shown that IgA1 protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B.
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