Evaluation of serum allergen‐specific IgE for the diagnosis of food adverse reactions in the dog
1998
Mueller, | Tsohalis,
A new monoclonal enzyme‐linked immunoassay (ELISA; CMG IMMUNODOT, Fribourg, Switzerland) measuring food antigen‐specific serum IgE was used in an attempt to investigate food allergen‐specific IgE in dogs. The serum of eight dogs with clinically proven adverse reactions to specific proteins was tested for beef, cow’s milk, pork, lamb, hen’s egg, soybean, fish mix (cod/sole), peanut, maize and wheat flour. The control group consisted of three healthy dogs, three dogs with nonallergic skin disease, two dogs with atopy, a cat and a horse. Only three mild positive reactions to beef, lamb and peanut, respectively, were found in this study; the sera were from two control dogs with the clinical diagnosis of dermatophytosis and atopy. None of the animals with confirmed food adverse reactions showed positive reactions. This study indicates that the diagnosis of food adverse reactions in the dog by measuring allergen‐specific IgE with the used mononuclear ELISA is unreliable.
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