A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-d-aspartate (NMDA) receptors
2022
Kwon, Nahyun | Lim, Chang Su | Lee, Dayoung | Ko, Gyeongju | Ha, Jeongsun | Cho, Moonyeon | Swamy, K. M. K. | Lee, Eun-Young | Lee, Dong-joon | Nam, Sang-Jip | Zhou, Xin | Kim, Hwan Myung | Yoon, Juyoung
Glutathione (GSH) is known to play a key role in the modulation of the redox environment in N-methyl-d-aspartate (NMDA) receptors. Coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties was synthesized and reported to monitor GSH near NMDA receptors. The cyanoacrylamide moiety allows probe 1 to monitor GSH reversibly at pH 7.4 and the ifenprodil group acts as a directing group for NMDA receptors. Two-photon fluorescence microscopy allows probe 1 to successfully sense endogenous GSH in neuronal cells and hippocampal tissues with excitation at 750 nm. Furthermore, the addition of H₂O₂ and GSH induced a decrease and an increase in fluorescence emission. Probe 1 can serve as a potential practical imaging tool to get important information on GSH in the brain.
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