Pseudomonas zhaodongensis sp. nov., isolated from saline and alkaline soils
2015
Zhang, Lei | Pan, Yuanyuan | Wang, Kaibiao | Zhang, Xiaoxia | Zhang, Cheng | Zhang, Shuang | Fu, Xiaowei | Jiang, Juquan
Strain NEAU-ST5-21 ᵀ was isolated from saline and alkaline soils in Zhaodong City, Heilongjiang Province, China. It was aerobic, Gram-stain-negative, rod-shaped and motile with a polar flagellum. It produced yellow–orange colonies with a smooth surface, and grew in the presence of 0–5 % (w/v) NaCl (optimum 0 %, w/v), at temperatures of 20–40 °C (optimum 28 °C) and at pH 7–11 (optimum pH 7). Phylogenetic analyses based on the separate 16S rRNA gene sequences and concatenated 16S rRNA, gyrB and rpoD gene sequences indicated that strain NEAU-ST5-21 ᵀ belongs to the genus Pseudomonas in the class Gammaproteobacteria . The most closely related species is Pseudomonas xanthomarina , whose type strain (KMM 1447 ᵀ) showed gene sequence similarities of 99.0 % for 16S rRNA, 81.8 % for gyrB and 85.0 % for rpoD with strain NEAU-ST5-21 ᵀ. DNA–DNA hybridization values between strain NEAU-ST5-21 ᵀ and P. xanthomarina DSM 18231 ᵀ, Pseudomonas kunmingensis CGMCC 1.12273 ᵀ, Pseudomonas stutzeri DSM 5190 ᵀ, Pseudomonas oleovorans subsp . lubricantis DSM 21016 ᵀ, Pseudomomas chengduensis CGMCC 2318 ᵀ, Pseudomonas alcaliphila DSM 17744 ᵀ and Pseudomonas toyotomiensis DSM 26169 ᵀ were 52±0 % to 25±2 %. The DNA G+C content of strain NEAU-ST5-21 ᵀ was 65 mol%. The major fatty acids (>10 %) were C ₁₈ : ₁ω7 c and/or C ₁₈ : ₁ω6 c , C ₁₆ : ₁ω7 c and/or C ₁₆ : ₁ω6 c and C ₁₆ : ₀, the predominant respiratory quinone was ubiquinone 9, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid, phosphatidylglycerol, one unknown aminolipid, one unknown lipid and a glycolipid. The proposed name is Pseudomonas zhaodongensis sp. nov., NEAU-ST5-21 ᵀ (= ACCC 06362 ᵀ = DSM 27559 ᵀ) being the type strain.
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