Polarization fluoroimmunoassay of the herbicide dichlorprop
1993
Sanchez, F.G. | Navas, A. | Alonso, F. | Lovillo, J.
A polarization fluorescence immunoassay has been developed for the screening of the herbicide dichlorprop [(+/-)-2-(2,4-dichlorophenoxy)propanoic acid]. The assay is based on the difference in fluorescence polarization between free and antibody-bound fluoresceinamine-labeled dichlorprop. Antibodies toward dichlorprop were produced by immunizing a rabbit with conjugates of dichlorprop to bovine serum albumin. Polarization measurements were performed by using two spectrofluorometers which use different optic configurations. Calibration curves fitted to an IC50 four-parameter logistic model gives chi-square 10(-5). The upper end of the standard curve for polarization fluorescence immunoassay is 0.01 micrograms/mL and the lower end 100 micrograms/mL. The method offers good analytical specifications and little cross-reactivity against similar compounds. The proposed method is compared with enzyme-linked immunosorbent assay using the same set of reagents. The effectiveness of the method on real samples was checked in spiked apple samples.
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