Molecular cloning, characterization of Pomacea canaliculata HSP40 and its expression analysis under temperature change
2019
Xu, Yipeng | Zheng, Guowan | Liu, Guangfu | Yang, Qian | Yu, Xiaoping
Heat shock proteins (HSPs) play important roles in the adaption of Pomacea canaliculata to unsuitable environments. In the present study, a cDNA encoding HSP40 in P. canaliculata (PocaHSP40) was cloned and characterized. The PocaHSP40 cDNA was 1466 bp, containing an ORF of 954 bp encoding 317 amino acids. Bioinformatics analysis showed that PocaHSP40 belonged to type II HSP40s and had four predicted phosphorylation sites. Phylogenetic analysis proved the conservation of HSP40s in mollusks. PocaHSP40 was widely expressed in the gill, digestive gland, kidney, and foot muscle of P. canaliculata. Challenged by different temperatures, the expression of PocaHSP40 was up-regulated under low temperatures but not high temperatures, which was contrary to the expression change of PocaHSP70 under low and high temperatures. These results implied that P. canaliculata evolved different strategies for survival under low temperature and high temperature through the regulation of HSPs.
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