A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids
2018
García‐Sarrió, María Jesús | Sanz, María Luz | Sanz, Jesús | González‐Coloma, Azucena | Cristina Soria, Ana
A new microwave‐assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box‐Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample: 10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid‐liquid extraction and ultrasound assisted extraction was also previously assessed. A comprehensive characterization of MAE extracts was carried out by GC‐MS. A total of 46 compounds, mostly terpenoids, were identified; piperitenone oxide and piperitenone were the major compounds determined. Several neophytadiene isomers were also detected for the first time in MAE extracts. Different procedures (solid‐phase extraction and activated charcoal (AC) treatment) were also evaluated for clean‐up of MAE extracts, with AC providing the highest enrichment in bioactive terpenoids. Finally, the MAE method here developed is shown as a green, fast, efficient and reproducible liquid extraction methodology to obtain M. rotundifolia bioactive extracts for further application, among others, as food preservatives.
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