The protein and peptide fractions of kashk, a traditional Middle East fermented dairy product
2020
Pourjoula, Mina | Picariello, Gianluca | Garro, Giuseppina | D'Auria, Giovanni | Nitride, Chiara | Rheza Ghaisari, Amid | Ferranti, Pasquale
Kashk is a typical dairy product of Iran, made from sour milk. It is traditionally produced from buttermilk in a dry, round-shaped form. Today, it is also produced at industrial level in a liquid form starting from fermented milk. We aimed to characterise the kashk proteome and peptidome comparing a traditional product with the industrial using a combination of proteomic approaches including advanced chromatographic and electrophoretic separation technique coupled to tandem mass spectrometry. We identified also phosphorylated casein-derived peptides (CPP) and investigated kashk protein digestibility using a static model of food protein digestion. The molecular characterization, coupled with bioinformatic in silico analysis, allowed the identification of potential bioactive peptides.
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