Phenylalanine Mutation to Cyclohexylalanine Facilitates Triangular Trimer Formation by β-Hairpins Derived from Aβ
2020
Haerianardakani, Sepehr | Kreutzer, Adam G. | Salveson, Patrick J. | Samdin, Tuan D. | Guaglianone, Gretchen E. | Nowick, James S.
Oligomers of the β-amyloid peptide, Aβ, play a central role in the pathogenesis and progression of Alzheimer’s disease. Trimers and higher-order oligomers composed of trimers are thought to be the most neurotoxic Aβ oligomers. To gain insights into the structure and assembly of Aβ oligomers, our laboratory has previously designed and synthesized macrocyclic peptides derived from Aβ₁₇–₂₃ and Aβ₃₀–₃₆ that fold to form β-hairpins and assemble to form trimers. In this study, we found that mutating Phe₂₀ to cyclohexylalanine (Cha) in macrocyclic Aβ-derived peptides promotes crystallization of an Aβ-derived peptide containing the Aβ₂₄–₂₉ loop (peptide 3F₂₀Cₕₐ) and permits elucidation of its structure and assembly by X-ray crystallography. X-ray crystallography shows that peptide 3F₂₀Cₕₐ forms a hexamer. X-ray crystallography and SDS-PAGE further show that trimer 4F₂₀Cₕₐ, a covalently stabilized trimer derived from peptide 3F₂₀Cₕₐ, forms a dodecamer. Size exclusion chromatography shows that trimer 4F₂₀Cₕₐ forms higher-order assemblies in solution. Trimer 4F₂₀Cₕₐ exhibits cytotoxicity against the neuroblastoma cell line SH-SY5Y. These studies demonstrate the use of the F20Cha mutation to further stabilize oligomers of Aβ-derived peptides that contain more of the native sequence and thus better mimic the oligomers formed by full-length Aβ.
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