The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters
1997
Block, M de | Debrouwer, D. | Moens, T.
Nuclear male sterility within Triticum aestivum is considered as the ideal basis for the development of a hybridization system for wheat. We engineered nuclear male sterility in wheat by introducing the barnase gene under the control of tapetum-specific promoters derived from corn and rice. A biolistic-mediated transformation method, based on the use of the poly(ADP-ribose)polymerase inhibitor niacinamide, was set up which enriched for low-copy integrations (1-3 copies). Most of these copies were not linked and segregated in the next generation.
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