High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species
2015
Inglin, Raffael C. | Stevens, Marc J.A. | Meile, Lukas | Lacroix, Christophe | Meile, L. (Leo)
We describe high-throughput screening techniques to rapidly detect either antimicrobial activity, using an agar-well diffusion assay in microtiter plates, or antifungal activity using an agar-spot assay in 24-well plates. 504 Lactobacillus isolates were screened with minimal laboratory equipment and screening rates of 2000–5000 individual antimicrobial interactions.
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