Sequence diversity of yeast 2 micromoles RAF gene and its co-evolution with STB and REP1
1991
Xiao, W. | Pelcher, L.E. | Rank, G.H.
Despite the extensive study of yeast 2 micromole plasmid, the exact function of plasmid-encoded RAF gene is not clear. Variants of 2 micromole plasmids from industrial Saccharomyces cerevisiae yeasts were isolated and characterized. Sequencing of RAF alleles revealed about 8% nucleotide and 10% amino acid diversities between 2 micromole variants of closely related strains. RAF sequence variations were correlated with STB-REP1 sequence diversity. We also used restriction fragment length polymorphism linkage to screen a large number of yeast strains from different fermentation industries. The results clearly show a tight linkage of STB-REP1-RAF variations. Thus, our observations suggest that plasmid-borne cis- and trans-acting elements co-evolved to form an optimal molecular parasite and that RAF may play a role in active plasmid partitioning.
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