Production of 3-phenyllactic acid and 4-hydroxyphenyllactic acid by Pediococcus acidilactici DSM 20284 fermentation
2012
Mu, Wanmeng | Yu, Shuhuai | Zhu, Lanjun | Jiang, Bo | Zhang, Tao
It was reported that 3-phenyllactic acid (PLA) and 4-hydroxyphenyllactic acid (HPLA) could be produced by Pediococcus acidilactici DSM 20284. PLA and HPLA concentrations were 0.65 and 0.25 mM when P. acidilactici DSM 20284 was grown in MRS broth for 36 h. In comparison with P. acidilactici DSM 20284 fermentation using MRS medium alone, PLA and HPLA production could be significantly promoted by the supplement of the corresponding amino acid (phenylalanine and tyrosine) and ketoacid (phenylpyruvic acid and 4-hydroxyphenylpyruvic acid) precursors in MRS broth, but inhibited by the supplement of the non-corresponding precursors. Promotion and inhibitory effects were both strengthened when supplement amounts increased. Ketoacids precursors showed more promotion effect on the corresponding 2-hydroxy acids production than amino acid precursors; however, amino acid precursors showed more inhibitory effect on non-corresponding 2-hydroxy acids production than ketoacids precursors.
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