Four spiroquinazoline alkaloids from Eupenicillium sp. isolated as an endophytic fungus from leaves of Murraya paniculata (Rutaceae)
2005
Barros, F.A.P. | Rodrigues-Filho, E.
A Eupenicillium spp. was isolated from healthy leaves of Murraya paniculata (Rutaceae) after surface sterilization. This fungus was cultivated in sterilized white-corn, where the spiroquinazoline alkaloids alanditrypinone, alantryphenone, alantrypinene and alantryleunone were produced. The culture was extracted with methanol and the alkaloids were isolated by a combination of silica gel chromatography and HPLC. The structures were elucidated by extensive spectroscopic studies, including 2D NMR and MS-MS experiments. The possible role of endophytes in the biosynthesis of plant secondary metabolites is discussed.
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