Purification of pediocin PA-1 by immunoaffinity chromatography
2008
Naghmouchi, Karim | Drider, Djamel | Fliss, Ismail
A one-step purification procedure for purifying pediocin PA-1, a class Ila bacteriocin produced by Pediococcus acidilactici UL5, was developed based on column immunoaffinity chromatography with specific antipediocin PA-1 polyclonal antibodies coupled to cyanogen bromide-activated Sepharose[TM]. About 13.3 [micro]g/mL purified pediocin PA-1 was obtained from 15 mL P. acidilactici UL5 culture supernatant, as measured by enzyme-linked immunosorbent assay. The specific activity and average recovery of the eluted pediocin PA-1 were about 6602 AU/mg and 53.3%, respectively. This is the first report of successful purification of pediocin PA-1 by immunoaffinity using pediocin PA-1-specific polyclonal antibodies.
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