Breeding of a non-urea-producing sake yeast carrying a FAS2 mutation

Breeding of a non-urea-producing sake yeast carrying a FAS2 mutation

2017

We isolated non-urea-producing sake yeasts from a FAS2-G3748A (G1250S) mutant using established positive-screening medium (containing canavanine, arginine and ornithine; CAO medium) after selection of FAS2 mutants with cerulenin. Varying levels of ethyl caproate production were observed due to spontaneous allelic recombination of the FAS2 heterozygotes on the CAO medium. The resulting double mutants exhibited favorable fermentation properties for sake brewing.

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AGROVOC Keywords

arginase arginine breeding brewing canavanine fermentation hexanoic acid mutants mutation ornithine yeasts

Bibliographic information

Published in

Mycoscience

Volume 58 Issue 4 Pagination 302 - 306 ISSN 1340-3540

Publisher

Elsevier Masson SAS

Other Subjects

Heterozygosity; Ethyl caproate; Cerulenin; Sake; Loss of heterozygosity; Ethyl carbamate; Car1

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Modification date: 2026-02-03

Format: MODS

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DOI https://dx.doi.org/10.1016/j.myc.2017.02.005

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Breeding of a non-urea-producing sake yeast carrying a FAS2 mutation

2017

AGROVOC Keywords

Bibliographic information