High-Sensitivity Naphthalene-Based Two-Photon Fluorescent Probe Suitable for Direct Bioimaging of H2S in Living Cells
2013
Mao, Guo-Jiang | Wei, Tian-Tian | Wang, Xu-Xiang | Huan, Shuang-yan | Lu, Dan-Qing | Zhang, Jing | Zhang, Xiao-Bing | Tan, Weihong | Shen, Guo-Li | Yu, Ru-Qin
H₂S is the third endogenously generated gaseous signaling compound and has also been known to involve a variety of physiological processes. To better understand its physiological and pathological functions, efficient methods for monitoring of H₂S in living systems are desired. Although quite a few one photon fluorescence probes have been reported for H₂S, two-photon (TP) probes are more favorable for intracellular imaging. In this work, by employing a donor-π-acceptor-structured naphthalene derivative as the two-photon fluorophore and an azide group as the recognition unit, we reported a new two-photon bioimaging probe 6-(benzo[d]thiazol-2′-yl)-2-azidonaphthalene (NHS1) for H₂S with improved sensitivity. The probe shows very low background fluorescence in the absence of H₂S. In the presence of H₂S, however, a significant enhancement for both one photon and TP excited fluorescence were observed, resulting in a high sensitivity to H₂S in aqueous solutions with a detection limit of 20 nM observed, much lower than the previously reported TP probe. The probe also exhibits a wide linear response concentration range (0–5 μM) to H₂S with high selectivity. All these features are favorable for direct monitoring of H₂S in complex biological samples. It was then applied for direct TP imaging of H₂S in living cells with satisfactory sensitivity, demonstrating its value of practical application in biological systems.
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