Immune-enhancing activity of polysaccharides isolated from Atractylodis macrocephalae Koidz
2015
Sun, Wenjing | Mȯngke, Bu. (Buġudan-u) | Qi, Chunhua | Yang, Xianyong | Wang, Yuge | Fan, Wentao | Yan, Zhengui | Zhao, Xiaona | Liu, Jianzhu
This study evaluated the immune-enhancing activity of polysaccharides from the rhizoma of Atractylodis macrocephalae Koidz (RAMPS) in vitro. Lymphocyte proliferation, cell cycle distribution, and percentages of CD4+ and CD8+ cells were determined. Different concentrations of RAMPS were added to peripheral blood T lymphocytes. Results showed that RAMPStp and RAMPS60c could significantly enhance T lymphocyte proliferation individually or synergistically with phytohemagglutinin at most concentrations. The active sites of RAMPStp and RAMPS60c were then selected. Lymphocyte cell cycle distribution and percentages of CD4+ and CD8+ cells were determined by flow cytometry. At most time points, RAMPS60c and RAMPStp could promote lymphocytes enter into S and G2/M phases. RAMPStp and RAMPS60c effectively improved the percentages of CD4+ and CD8+ T cells. RAMPStp produced optimal effects. Therefore, RAMPStp could be used as a component of novel immunopotentiators.
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