Transcriptome response of previtellogenic ovary in Anguilla japonica after artificial hormone injection
2017
Lee, Shang-Chien | Hsiao, Chung-Der | Wang, Yung-Song | Lou, Show-Wan
In this study we investigate the intra-ovarian pathways underlying early follicle development in Japanese eels, Anguilla japonica. We conducted high-throughput transcriptome analyses in the initial development of the ovary via the next-generation sequencing (NGS). Japanese eels were treated with three weekly salmon pituitary homogenate (SPH) injections. Using RNA-seq, we obtained 29,117,237 and 41,867,557 reads from the control and the SPH-injected groups, respectively. Combining these RNA-seq datasets, we acquired a total of 101,711 unigenes (N50=1,517bp) after performing de novo assembly. After differentially expressed gene (DEG) analysis, 4,211 and 7,059 annotated genes showed upregulation and downregulation respectively in SPH-injected ovarian tissues. Furthermore, functions of annotated genes were classified by GO and KEGG analyses. The PTEN/PI3K-Akt pathway, Tsc/mTOR signaling, oocyte meiosis and reproduction functions were found in data of differentially expressed genes.
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