Phenotypic effects of isolated pRiA4 TL-DNA rol genes in the presence of intact TR-DNA in transgenic plants of Solanum dulcamara L
1991
McInnes, E. | Morgan, A.J. | Mulligan, B.J. | Davey, M.R.
The effect of the rol genes, together with the TR-DNA of pRiA4 on the phenotype of Solanum dulcamara plants, was analysed. Plants transformed by Agrobacterium strain BN1010::rolA (rolA+TR+) exhibited severe leaf wrinkling, whereas plants transformed by strain BN1010::rolC (rolC+TR+) had a typical 'hairy root' phenotype. Leaf discs excised from these latter plants produced roots on hormone-free medium. BN1010::rolABC (rolABC+TR+) transformed plants had an exaggerated transformed phenotype. Some of the BN1010::rolABC transformants had positively geotropic root growth which correlated with the presence of multiple copies of the TR-DNA. S. dulcamara plants, transformed by the TR-DNA region only, exhibited epinasty. Scanning electron microscopy of plants containing various regions of agropine Ri T-DNA revealed that transformation causes changes in basic plant structure.
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