Growth and alkaloid production of a cell suspension culture of Thalictrum rugosum in shake flasks and membrane-stirrer reactors with bubble free aeration
1988
Piehl, G.W. | Berlin, J. | Mollenschott, C. | Lehman, J.
A novel membrane-stirrer reactor allowing bubble-free aeration was used for the cultivation of a cell suspension culture of Thalictrum rugosum. The immediate advantages were the absence of any foaming or flotation and the gentle mixing of the suspension by the tumbling movement of a coil or basket of membranes. In a 211 reactor under these conditions the suspensions were grown to high cell densities of 550 g fresh mass or 50 g dry mass/l. A pO2 of 30% was maintained up to 400 g/l without air bubble formation at the gas-exchange membranes. Provided that the physiological states of the initial suspensions were comparable, growth rates and alkaloid production of shake-flask and fermenter-grown cells were rather similar.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library