Homogenous liquid-liquid microextraction for determination of betulinic acid in the food samples using RP-HPLC
2013
Khajeh, Mostafa | Moghaddam, Mansour Ghaffari | Danesh, Atousa Zafar
In this work, a new, simple and fast homogenous liquid-liquid microextraction (HLLME) coupled with reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the determination of betulinic acid in food samples. The effect of various parameters on the extraction step including type and volume of extraction solvents, extraction time and ionic strength were studied and optimized. The results showed that the amount of ionic strength and extraction time were not affective on the extraction efficiency. In the optimum conditions, the limit of detection (LOD) and enrichment factor were 1.8 μgL⁻¹ and 110, respectively. Furthermore, the relative standard deviation of the ten replicate was <3.9%. The developed procedure was then applied to the extraction and determination of betulinic acid in the food samples.
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