ELISA and HPLC methods for analysis of fumagillin and its decomposition products in honey
1991
Assil, H.I. | Sporns, P.
Two sensitive methods for detection of the antibiotic fumagillin (winter honey bee medication used to control Nosema apis) in honey were developed. A reversed-phase high-performance liquid chromatography (HPLC) method could detect the presence of fumagillin in honey at levels of 100 ppb and was useful to identify decomposition products of fumagillin. An enzyme-linked immunosorbent assay (ELISA) was developed as an initial screening method for fumagillin with detection levels of at least 20 ppb of fumagillin in honey. Neofumagillin, which could also be detected by ELISA, was identified as the major product of light decomposition of fumagillin in honey. Fumagillin was very stable in honey at elevated temperatures (stable for at least 35 days at 80 degrees C). At the above detection limits, there was no evidence of fumagillin or its breakdown products found in any producer honey examined, including honey samples from a beekeeper who regularly used fumagillin.
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