Crystallization and preliminary X-ray crystallographic studies of thermostable xylanase crystals isolated from Paecilomyces varioti
1994
Eswaramoorthy, S. | Vithayathil, P.J. | Viswamitra, M.A.
A highly thermostable xylanase isolated from the thermophilic fungus Paecilomyces varioti has been crystallized by the vapour diffusion method. The isolation of this enzyme by crystallization directly from the culture filtrate projects this fungus as an important source for large-scale production of pure xylanase. The crystals belong to orthorhombic space group P212121 with the unit cell dimensions a = 38.48 angstroms, b = 53.87 angstroms and c = 90.23 angstroms. Four molecules occupy a volume of 187,039.4 cubic angstroms along with 34% of solvent. The data collected with an area detector to the resolution of 2.7 angstroms were used to calculate the unit cell parameters and Matthews' constant. The optical behaviour of the crystal was studied at different temperatures to understand its thermal stability.
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