Optimization of multi-stage countercurrent extraction of antioxidants from Ginkgo biloba L. leaves
2012
Yu, Chen-huan | Chen, Jing | Xiong, Yao-kang | Li, Xiao-xian | Dai, Xiao-yan | Shi, Chang-cheng
Multi-stage countercurrent extraction (MCE) as a novel extraction technique was used to extract antioxidants from Ginkgo biloba leaves. Orthogonal array design (OAD) was employed to optimize the ratio of 60% ethanol to raw material (8–16mL/g), extraction time (30–60min) and extraction temperature (60–80°C) to obtain a high yield of antioxidants from G. biloba leaves by MCE. The optimum conditions were a ratio of 60% ethanol to raw material of 16mL/g and extraction time of 30min at 80°C. Under these conditions, the yields of flavonoids and total phenolics were 1.74% and 2.42%, respectively, and DPPH radicals scavenging activity of the extract was 89.97%. Compared with heat-reflux extraction, MCE had obvious advantages of less extraction time and lower solvent and energy consumption. It may be used as a promising technique for the extraction of bioactive compounds from plant materials.
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