Various Specificities of the DNA Dependent RNA Polymerase for Synthetic Templates
2014
Matzura, Hans
The transcription of the homopolymer templates poly dA: dT and poly dG: dC with RNA polymerase is described. In both cases the pyrimidine strands are transcribed preferably, as shown by studies in which only one or both of the triphosphates complementary to the strands were present as substrates. A poly dA: dT dependent poly A synthesis which had reached a plateau could no longer be stimulated by addition of new poly dA: dT. If instead poly dG: dC and GTP were added, the beginning of a poly G synthesis could be observed.
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