Oceanospirillum sediminis sp. nov., Isolated From Coastal Sediment in the Yellow Sea
2022
Zhao, Huilin | Tian, Yue | Sun, Xunke | Wu, Qianwen | Chen, Si | Shan, Jiangfan | Zhong, Yu | Chen, Xinyu | Gao, Xiaoxue | Liu, Shengnan | Wang, Ruochen | Du, Zongjun | Li, Boqing | Ji, Xiaofei
A novel Gram-negative, motile, aerobic, spiral-shaped bacterium designated D5ᵀ, was isolated from a coastal sediment collected in the Yellow Sea. Optimal growth occurred at 30 °C, pH 7.0–8.0 and in the presence of 1–3% (w/v) NaCl. Strain D5ᵀ contained ubiquinone 8 (Q-8) as the predominant respiratory quinone. The major fatty acids (> 10%) were C₁₆:₀, C₁₆:₁ ω7c/C₁₆:₁ ω6c and C₁₈:₁w7c/C₁₈:₁w6c. The main polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The draft genome is 5.6 Mb in length, and DNA G + C content is 47.2 mol%. 16S rRNA gene sequences showed that strain D5ᵀ is most closely related to Oceanospirillum beijerinckii NBRC 15445ᵀ (97.8%, sequence similarity). However, the digital DNA-DNA hybridization (dDDH) value and average nucleotide identity (ANI) between strain D5ᵀ and O. beijerinckii is only 27.8% and 77.1%. Phylogenetic trees based on 16S rRNA gene sequences and whole genomes all indicated that strain D5ᵀ formed a separate branch in the genus Oceanospirillum. Combined results of the polyphasic analyses suggested that strain D5ᵀ represents a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sediminis sp. nov. is proposed. The type strain is D5ᵀ (= MCCC 1K06061ᵀ = KCTC 62987ᵀ).
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