High-throughput chemiluminescence immunoassay based on Co2+/hemin synergistic catalysis for sensitive detection tetrabromobisphenol A bis(2-hydroxyethyl) ether in the environments
2020
Gu, Lantian | Zou, Yanmin | Li, Yanshen | Zeng, Kun | Zhu, Nuanfei | Zhu, Fang | Gyimah, Eric | Yakubu, Salome | Meng, Hui | Zhang, Zhen
Here, a novel chemiluminescence (CL) immunoassay was fabricated for sensitive determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE), one of typical tetrabromobisphenol A derivatives. At the indirectly competitive method, the synthesized PS@hemin@Co²⁺ was labelled by secondary antibody (Ab₂) instead of common natural enzymes, which showed excellent catalysis towards the decomposition of luminol-H₂O₂ for producing CL signal. Furthermore, the CL signal was greatly amplified owing to the synergistic catalysis of hemin and Co²⁺ in the detection system. Under the optimized conditions, the established method offered (i) low detection limit (LOD, 0.9 μg/L), which was almost 5 times lower than that using a conventional ELISA with the same antibody; (ii) a good linearity (1.6–14.3 μg/L); (iii) satisfactory accuracy and precision (recoveries, 89.67–125.33%; CV, 2.75–8.37%). The proposed CL immunoassay was applied for analysis of environmental samples from various sources collected from Jiangsu and Zhejiang province, China. And the detected concentrations were ranged in 2.4–3.7 μg/L in environmental waters and 1.8–2.4 ng/g (dry weight, dw) in soil samples, indicating great potential for trace TBBPA-DHEE detection from environmental samples.
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